منابع مشابه
The Molecular Weight of Crystalline Catalase.
In 1933 Stern (2) used the diffusion method of Northrop and Anson (3) to determine the molecular weights of purified catalase from horse liver, pig liver, cow liver, horse kidney, and cow erythrocytes. He found the average value for the molecular weight of catalase to be 68,900, and stated that the catalase molecule has a dimension similar to that of hemoglobin. In 1938 Stern and Wyckoff (4) pu...
متن کاملCrystalline bacterial penicillinase.
Church, B. D. (1955). The role of L-alanine and glucose on dormancy in spores of aerobic bacteria. D.Phil. Thesis: University of Michigan. Conway, E. J. (1947). Microdiffusion Analysi8 and Volumetric Error. London: Lockwood. Crook, P. G. (1952). J. Bact. 63, 193. Gunsalus, C. F. & Tonzitich, J. (1952). Nature, Lond., 170, 162. Hagedom, J. C. & Jensen, B. N. (1923). Biochem. Z. 136, 46. Henry, B...
متن کاملPreparation of crystalline erythrocuprein and catalase from human erythrocytes.
The removal of hemoglobin from blood cell lysates used for the preparation of various erythrocyte enzymes has been commonly achieved by denaturation through the use of a chloroform-ethanol mixture, the so-called Tsuchihashi procedure (1). Although there has been speculation concerning possible modification of the nonhemoglobin protein by such treatment (2, 3), no clear cut, alternative, large s...
متن کاملPreparation of Crystalline Erythocuprein and Catalase from Human Erythrocytes*
The removal of hemoglobin from blood cell lysates used for the preparation of various erythrocyte enzymes has been commonly achieved by denaturation through the use of a chloroform-ethanol mixture, the so-called Tsuchihashi procedure (1). Although there has been speculation concerning possible modification of the nonhemoglobin protein by such treatment (2, 3), no clear cut, alternative, large s...
متن کاملA solubility analysis of crystalline ox-liver catalase.
Sedimentation. This was observed in a Svedberg oilturbine ultracentrifuge by the method of Cecil & Ogston (1948). The hydrochlorides were dissolved in 0-2M-NaCI to a concentration of 1 g./100 ml. Owing to the relatively small molecular weights and high diffusion coefficients, clear sedimentation boundaries were not obtained; the sedimentation constants were therefore measured bythe method ofGut...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1948
ISSN: 0306-3283
DOI: 10.1042/bj0430193